Generator

Part:BBa_K1741006:Design

Designed by: Marta Żardecka   Group: iGEM15_UAM_Poznan   (2015-09-17)


sfGFP under short rhamnose promoter (very weak)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 148


Design Notes

We removed both CPR sites.


Source

All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.

References